Mouse p53 purification
Nettetp53 Protein Overview The transcription factor p53 responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, … NettetClone PAb 240. Western blot analysis of p53. Lysate from SV40-transformed COS-7 cells were probed with anti-p53 (Cat. No. 554166). p53 is identified as a band of 53 kDa. Reactivity: Human, Mouse, Rat, Monkey, Hamster, Chicken, Cow (QC Testing) Application: Western blot (Routinely Tested), Immunohistochemistry-frozen, …
Mouse p53 purification
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NettetMouse p53 Antibody Summary Species Reactivity Mouse Specificity Detects mouse p53 in Western blots. Source Monoclonal Rat IgG 2B Clone # 197643 Purification Protein A or G purified from hybridoma culture supernatant Immunogen E. coli -derived recombinant mouse p53 Leu28-Phe325 Accession # NP_035770 Formulation Nettet12. nov. 2024 · The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased …
Nettet22. feb. 2024 · The p53 isoform p53/47 (p47 or ΔNp53) is linked to aging and neural degeneration and is expressed in human cells via an alternative cap-independent translation initiation from the 2nd in-frame... NettetPurified Mouse Anti-Human p53 Clone DO-7 (RUO) View all Formats Anti-human p53, Cat. No. 554294. Formalin-fixed, paraffin-embedded tissue section of human breast …
Nettet22. mar. 2024 · Protein purification Recombinant p53 was purified using a drip column containing 5 mL of nickel-nitrilotriacetic acid (Ni 2+ -NTA) resin (Qiagen) [ 28 ]. This … NettetThe importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, …
Nettet14. sep. 2016 · Moreover, loss of the interaction with SET activates p53, resulting in tumour regression in mouse xenograft models. Notably, the acidic domain of SET acts as a ‘reader’ for the unacetylated ...
NettetPVDF membrane was probed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # HAF1355). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. t9 adversary\u0027sNettet4. okt. 2016 · In conclusion, we have identified a mouse p53 acetylation at K98 lysine (K101 for human p53) that is crucial for the regulation of p53 metabolic targets, and … t9 Joseph\u0027s-coatNettet10. apr. 2024 · sST2 inhibits MCFs senescence and Sirt1/p53/p21 signaling, accelerating cardiac fibrosis in VMC mice. The heart tissues were collected on day 15 for … t9 anarchist\u0027sNettetWild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 … t9 30watt fluorescent replacement bulbNettet7. apr. 2024 · 6SHFLHV5HDFWLYLW\ Human/Mouse/Rat 6SHFLILFLW\ Detects human, mouse, and rat p53. 6RXUFH Polyclonal Goat IgG 3XULILFDWLRQ Antigen Affinitypurified ,PPXQRJHQ ( FROLderived recombinant human p53 Asp7Asp393 Accession # P04637)RUPXODWLRQ Lyophilized from a 0.2 μm filtered solution in … t9 aspect\u0027sNettet12. nov. 2024 · Immunocytochemistry. p53 in HeLa Human Cell Line. p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the … t9 40wNettetPurified Mouse Anti-Human p53 Clone G59-12 (RUO) View all Formats Western blot analysis of p53. A SV-40 transformed rat granulosa cell lysate was probed with anti-human p53 (clone G59-12, Cat. No. 554157) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). Clone G59-12 identifies p53 at 53 kDa. Show More Spectrum Viewer t9 acknowledgment\u0027s