WebbThermo Scientific™ Phire™ Green Hot Start II DNA Polymerase is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Reaction Buffer. Phire Hot Start II DNA … WebbNote: Phire Hot Start DNA Polymerase produces blunt end. 3. Guidelines for using Phire Hot Start II DNA Polymerase Phire Hot Start II DNA Polymerase is provided with 5X Phire Green Reaction Buffer. The buffer contains 1.5 mM MgCl2 at final reaction concentration. A separate tube of DMSO is provided for further optimization. 3.1.
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Webb30 mars 2024 · Proteolytic enzymes are commercially valuable and have multiple applications in various industrial sectors. The most studied proteolytic enzymes produced by Yarrowia lipolytica, extracellular alkaline protease (Aep) and extracellular acid protease (Axp), were shown to be good candidates for different biotechnological applications. In … Webb5X Phire Green Reaction Buffer 4 1.5 mL 50 mM MgCl 2 2 1.5 mL 100% DMSO 0.5 mL www.thermofisher.com For Research Use Only. Not for use in diagnostic procedures. … describe hot warm and cold backup sites
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