Phospho-akt1/2/3 ser473 cellular kit

Author: ukre

August undefined, 2024

WebPhospho-Akt (Ser473) Antibody detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues. It does not recognize Akt phosphorylated at other sites, nor does it recognize phosphorylated forms of related kinases such as PKC or … WebMyricetin ameliorates bleomycin-induced pulmonary fibrosis in mice by inhibiting TGF-β signaling via targeting HSP90β

Phospho-Akt (Ser473) Kit - Meso Scale

WebMMP1 Antibody SKU: A... WebApr 14, 2024 · Notably, when proline in the 14-3-3 binding motif was replaced with an alanine (P436A), the 14-3-3 recognition of SIK1 was profoundly attenuated (Supplementary Fig. S4G). Together, these data suggest that AKT-mediated SIK1 phosphorylation enhances SIK1 binding with 14-3-3, which in turn increases the rate of SIK1 transfer to the cytoplasm. city of sanford nc phone number

Total SHP1 cellular HTRF kit Cisbio

LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells. Web(Ser473) Chemiluminescent Sandwich ELISA Kit detects endogenous levels of phospho-Akt1 (Ser473) in human, rat and mouse cells. Please visit www.cellsignal.com for a complete listing of recommended companion products. #7134C PathScan® Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit Species Cross-Reactivity: H, M, R … WebBath Filler and Heat Transfer Fluids; Bath Specific Platforms and Clamps; Bath Specific Tube Racks; Calibration Baths; Circulating Baths; Coliform Baths dos in group discussion

Phospho-AKT1/2/3 (Ser473) cellular kit, 10000 Tests - FYI-Sandbox

PathScan® Phospho-Akt1 (Ser473) Sandwich ELISA Kit

WebThe Total SHP1 cellular assay monitors the expression level of SHP1, and can be used as a normalization assay with our phospho-SHP1 Y564 kit. Because these phospho and total SHP1 assays are compatible, the two kits can be used in parallel on the same lysates. Many cancer cells overexpress checkpoint inhibitor ligands such as PD-L1. WebThe protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents Product specifications Designed with signal performance in mind, the kit offers a tailored standard curve ideally suited to the study of Gi coupled GPCRs. Introduction to product validation city of sanford nc logoWebProduct Note This antibody is raised against human AKT1 phosphorylated at Ser473. Based on sequence homology, it is predicted to react with AKT2 and AKT3 when phosphorylated at the corresponding residues - AKT2 Ser474 and AKT3 Ser472. Predict Reactivity Zebrafish, Sheep, Bovine, Cat, Chicken, Rhesus Monkey (>80% identity) PROPERTIES TARGET city of sanford nc job openings

"Web#IR172-668 / IR172-668,Phospho-AKT (Ser473) antibody. Products ... and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. ... Subsequent phosphorylation of both threonine residue 308 and serine ... " - Phospho-akt1/2/3 ser473 cellular kit

Phospho-akt1/2/3 ser473 cellular kit

Phosphatidylinositol 3-kinase (PI3K) pathway activation in bladder ...

WebThe Phospho-AKT3 (Ser473) kit is designed for the robust quantification of AKT3 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), AKT is an … WebMMP1 Antibody SKU: A...

Did you know?

WebPhospho-AKT1 (Ser473) Antibody (700392) in ICC/IF Immunofluorescent analysis of AKT (pS473) was done on 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. Webphospho-AKT 1/2/3 (Ser473) Detection Antibody Reagent Positive Control Cell Lysate (lyophilized) Detection Reagent (TMB Substrate) Stop Solution Plate seal Description …

WebThis HTRF kit is designed to monitor AKT1 phosphorylation on Ser473 as a readout of Pi3K pathway ... Learn More Phospho-AKT1/2/3 (Ser473) cellular kit WebThe Alpha SureFire® Ultra™ HV Multiplex p-AKT 1/2/3 615 (Eu) assay kit is used to measure the phosphorylation of AKT1/2/3 (Ser473) plus another target of choice from the AlphaLISA SureFire ... Akt (1/2/3) at the phospho-Ser473 epitope, and one at a …

WebAKT, also known as protein kinase B, is an important regulator of numerous cellular processes including cell survival, glucose metabolism, transcription, and apoptosis. AKT … WebFeb 3, 2024 · Protein kinase B (AKT1) is hyper-activated in diverse human tumors. AKT1 is activated by phosphorylation at two key regulatory sites, Thr308 and Ser473. Active AKT1 phosphorylates many, perhaps hundreds, of downstream cellular targets in the cytosol and nucleus. AKT1 is well-known for phosphorylatin …

WebPhospho-AKT1/2/3 (Ser473) control lysate HTRF®. Phospho-AKT1/2/3 (Ser473) control lysate. This lysate is a component of the phospho-AKT1/2/3 (Ser473) kit. It may be used …

WebAlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kits, PerkinElmer Supplier: Perkin Elmer Harmonized Code US-3822005090 The AlphaLISA® SureFire® Ultra™ p-AKT1/2/3 … dosing schedule for shingles vaccine city of sanford nc police reportWebUp-regulation of O-GlcNAcylation increases Akt1 phosphorylation at Ser473. The 8305C cells were treated with 5 μM Thia-G or were transfected with 500 ng OGT plasmid, and then cell lysates were resolved by SDS-PAGE, and phospho-Akt1 (Ser473) or Akt1 were determined with specific antibodies (Figure 4A and B). An increase in Ser473 ... city of sanford nc mulchWebThe FastScan™ Phospho-Akt (Ser473) ELISA Kit detects endogenous levels of Akt when phosphorylated at Ser473 as shown in Figure 1. This kit detects proteins from the … dosing schedule for tremfyaWebAlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kits, PerkinElmer Supplier: Perkin Elmer Harmonized Code US-3822005090 The AlphaLISA® SureFire® Ultra™ p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Order Now … city of sanford nc police departmentWebAKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of … dosing seachem ironWebPhospho-Akt (Ser473) Rabbit mAb has been coated on the microwells. After incubation with cell lysates, phospho-Akt (Ser473) protein is captured by the coated antibody. Following extensive washing, Akt1 Mouse Antibody is added to detect the captured phospho-Akt1 (Ser473) protein. dosing schedule of mounjaro